Figure 5
From: Single-cell qPCR on dispersed primary pituitary cells -an optimized protocol
Efficiency of SYBR green I assays using specific primers for cod follicle-stimulating hormone beta subunit ( FSHβ ), luteinizing hormone beta subunit ( LHβ ), and elongation factor 1 α ( EF1α ). A-C A three-fold cDNA (made from 3 μg of cod pituitary total RNA) template dilution curve from 1:20 to 14580 (linearity down to 1620 times for LHβ) was made for each primer pair tested. The crossing point (Cq; ordinate) values are plotted against the relative logarithmic concentration (abscissa) of the cDNA in the initial solution. The slope of the regression line is then used to calculate the efficiency of the qPCR assay. The efficiency was 2.020 for the FSHβ primer pair, 1.996 for the LHβ primer pair, and 2.005 for the EF1α primer pair.