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Figure 4 | BMC Molecular Biology

Figure 4

From: Single-cell qPCR on dispersed primary pituitary cells -an optimized protocol

Figure 4

Efficiency of the SYBR green I assay using specific primers for rat prolactin ( PRL ). A. Primer pairs that did not amplify genomic DNA were further tested using a dilution curve with three-fold cDNA (made from 10 000 GH4 cells) template dilutions between 1:20 and 1:14580. The crossing point (Cq; ordinate) values are plotted against the relative logarithmic concentration (abscissa) of the cDNA in the initial solution. The slope of the regression line is then used to calculate the efficiency of the qPCR assay. The assay for rat PRL had an efficiency of 1.998, equivalent to about 100%.

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