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Figure 8 | BMC Molecular Biology

Figure 8

From: Regulation of PURA gene transcription by three promoters generating distinctly spliced 5-prime leaders: a novel means of fine control over tissue specificity and viral signals

Figure 8

MCMV infection suppresses PURA transcription and reduces levels of Purα protein. A. The locations within the PURA gene of four sequences amplified by the indicated primers (Table 1). B. The relative concentrations of transcript downstream of TSS II in MCMV infected NIH-3T3 cells as determined by rt-PCR are shown relative to concentrations of transcript in mock treated cells (see Methods). C. Proteins, isolated from the MCMV infected 3T3 cell lysates used in panel B, were assayed for Purα by immunobloting. β actin was used as a loading control.

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