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Figure 5 | BMC Molecular Biology

Figure 5

From: Regulation of PURA gene transcription by three promoters generating distinctly spliced 5-prime leaders: a novel means of fine control over tissue specificity and viral signals

Figure 5

Relative abundance of PURA transcripts in a panel of eight human tissues. RT-PCR was performed with 8 human cDNAs using the indicated PURA specific primers to amplify sequences downstream of TSS II (TSS II-1, TSS II-2, and TSS II-3) or within the coding sequence (cds) (see Figure 2A) as described in Methods. Primers specific to glyceraldehyde 3-phosphate dehydrogenase (G3PDH) were used to estimate loading. Results are given as the difference in Ct values (ΔCt) determined by RT-PCR for a designated amplicon and a control; ΔCt = Ct (cds) - Ct (G3PDH), left panel; ΔCt = Ct (TSSII-1) or Ct (TSSII-2) or Ct (TSSII-3) - Ct (cds), right panel. Note that a higher Ct indicates a lower level of transcript by log to the base 2. Ct values should be seen as indicating the relative abundance of cDNA amplified by each primer pair and not a quantitative estimate of the difference in abundance of the cDNA sequences amplified in a particular tissue.

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BMC Molecular Biology

ISSN: 1471-2199

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