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Figure 4 | BMC Molecular Biology

Figure 4

From: Regulation of PURA gene transcription by three promoters generating distinctly spliced 5-prime leaders: a novel means of fine control over tissue specificity and viral signals

Figure 4

Transfection of the TSS II PURA promoter and promoter deletion constructs demonstrates that sequences within the promoter are important for PURA transcription. A. The 2554 bp PURA sequence containing TSS II and III as cloned into the pGL3 reporter vector is shown with transcription factor binding elements, MatInspector (Genomatrix). An identified series of deletion mutants is shown below. A, B. NCI- H82 and NCI-H146 cells were cotransfected in triplicate with 1.0 μg of the indicated constructs and 0.1 μg pCMV-RL to correct for transfection efficiency, as described in Methods. Forty-eight hours post-transfection cell lysates were prepared for analysis of luciferase expression (see Methods). Numbers indicate transcription from pGL3-PURAPr constructs normalized to transcription from pGL3-BV vector. All statistical analyses were done with two-way ANOVA using the Bonferroni posttest as conducted using Prism 4.0. ***, P < 0.001.

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BMC Molecular Biology

ISSN: 1471-2199

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