Figure 2

Multiple sequence alignment of MOF and related MYST HAT domains. Alignment calculated with T-Coffee (Notredame et al., 2000). Figure prepared with Espript (Gouet et al, 1999). N-terminal sequences including chromodomains were removed manually after the initial alignment was completed. Secondary structures derived from the atomic coordinates of the hMOF HAT domain (PDB ID 2PQ8; Temple, W. et al. Structural Genomics Consortium), and colored according to the domain representations depicted in Figure 3. Active site residues mutated in dMOF in this study are marked by blue stars. Zinc finger ligand residues are marked by orange triangles. Moderately conserved residues are boxed and have a yellow background. Strictly conserved residues are boxed with a red background. The sequences of the HAT domains are: Human_MOF (Homo sapiens MOF, gi14149875), Droso_MOF (Drosophila melanogaster MOF, gi3024151); Zebrafish_MOF (Danio rerio Hat1, gi 160774364); Droso_Tip60 (Drosophila melanogaster Tip60, gi18858193); Human Tip60 (Homo sapiens Tip60, gi 12652827); Zebraf_Tip60 (Danio rerio Tip60, gi225543380); Yeast Esa1 (Saccaromyces cerevisiae Esa1, gi3023717); Yeast_SAS2 (Saccaromyces cerevisiae SAS2, gi730713); Kluyver_SAS2 (Kluyveromyces lactis SAS2 gi50311675); Ashbya_SAS2 (Ashbya gossypii SAS2 homologue, gi45198739).