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Table 2 The 442 perennial ryegrass samples analysed during the study and which datasets they were included in.

From: Validation of reference genes for quantitative RT-PCR studies of gene expression in perennial ryegrass (Lolium perenne L.)

Experiment

Tissue type

Number of treatments

Biological replicates

Sampling dates

Total number of samples (treatments × replicates × dates)

Datasets included in1

Defoliation management

Leaf

6

9 (3 spatial × 3 temporal)

4

2062

A, B, E

 

Stubble

6

9 (3 spatial × 3 temporal)

4

216

A, B, D

Cultivar

Leaf

5

1

1

5

A, C, E, G

Seasonal3

Leaf

4

1

1

4

A, E, H

Moisture-stress

Leaf

3

1

1

3

A, C, E, I

Cold-stress

Leaf

2

1

1

2

A, C, E, J

 

Stubble

2

1

1

2

A, C, D, J

Other

Inflorescence

1

1

2

2

A, F

 

Roots

1

1

1

1

A, F

 

Callus

1

1

1

1

A, C, F

Total number of samples 4

   

442

 
  1. 1Datasets consist of (A) all 442 perennial ryegrass tissue samples, (B) 422 field-grown samples harvested following different defoliation management, (C) 13 laboratory-grown samples, (D) 218 perennial ryegrass stubble samples, (E) 220 perennial ryegrass leaf samples, (F) four perennial ryegrass callus, inflorescence and root samples, (G) five perennial ryegrass etiolated seedlings of different cultivars, (H) four field-grown samples harvested at the peak of each season, (I) three laboratory-grown samples to evaluate water stress and (J) four laboratory-grown samples to evaluate cold stress.
  2. 2There were 216 leaf samples in total, but in 10 of the samples taken immediately after defoliation there was insufficient leaf for RNA extraction.
  3. 3The four seasonal samples (autumn, winter, spring and summer) each consisted of two original tissue samples (one collected pre-grazing and one collected post-grazing at the peak of each season) that were bulked together after cDNA synthesis.
  4. 4Each of the 442 samples was tested in triplicate using qRT-PCR.