Validation of reference genes for quantitative RT-PCR studies of gene expression in perennial ryegrass (Lolium perenne L.)

Table 1 The Serial Analysis of Gene Expression (SAGE™) tags and normalised copy numbers for candidate reference genes found in field-grown perennial ryegrass tissue sourced from pre- and post-grazed swards.

Gene abbreviation	Gene name	Accession numbers		SAGE™ tag³	Copies per 100,000 transcripts
		dbEST ¹	TSA ²		Winter	Spring	Summer	Autumn
eEF1A (h)	Eukaryotic elongation factor 1 alpha	GO924753	N/A⁴	CTATGTTCGA	68	87	161	102
eEF1A (s)	Eukaryotic elongation factor 1 alpha	GO924806, GO924801, GO924766, GO924798, GO924804	EZ421973	CTATGTTCGG	41	47	37	31
TBP-1	26S proteasome regulatory subunit 6A homolog	GO924783, GO924768, GO924761, GO924782, GO924758, GO924762	EZ421974	ATAATATGAA	11	13	43	29
eIF4A	Eukaryotic initiation factor 4 alpha	GO924770	N/A	TAAAACACTG	14	17	0	8
YT521-B	YT521-B-like family protein	GO924780, GO924796, GO924779, GO924805, GO924799, GO924765, GO924767	EZ421977	GAAGGTGGCT	20	10	6	2
H3	Histone 3	GO924769, GO924763, GO924764	EZ421975	AACTACTAAT	16	7	6	6
E2	Ubiquitin-conjugating enzyme	GO924794, GO924791	EZ421976	ATTTGGTTGA	5	13	0	2

¹National Centre for Biotechnology Information GenBank dbEST accession number/s for the perennial ryegrass sequence/s to which the tags are mapped.
²National Centre for Biotechnology Information GenBank TSA accession number for the perennial ryegrass sequence to which the tags are mapped.
³Tags are presented as a 10 base pair sequence, excluding the Nla III site [CATG; [21]].
⁴Not applicable.

ISSN: 1471-2199