Figure 4From: Validation of reference genes for quantitative RT-PCR studies of gene expression in perennial ryegrass (Lolium perenne L.)Relative quantification of the target gene EF-Tu in perennial ryegrass leaf tissue throughout regrowth. Normalisation was carried out using the four most stable reference genes defined by geNorm, the two most stable reference genes defined by NormFinder or the least stable reference gene, eEF1A (h).Back to article page