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Table 2 mRNA levels of CEACAM1 after silencing of IRF-1, IRF-2, USF1 and USF2

From: Regulation of CEACAM1 transcription in human breast epithelial cells

Treatment

Relative amount 1

Percent of control 2

None

1.4 ± 0.1

93

Lipofectamine

1.4 ±0.1

93

Negative control3

1.5 ±0.1

100

IRF1 #1

0.9 ±0.1 *

60

IRF1 #2

0.8 ±0.2 *

53

IRF1 #3

1.3 ±0.1

87

IRF2 #1

1.4 ±0.1

93

IRF2 #2

1.0 ±0.2 *

67

IRF2 #3

0.9 ±0.1 *

60

USF1 #1

1.1 ±0.2 *

73

USF1 #2

1.1 ±0.2 *

73

USF1 #3

1.0 ±0.1 *

67

USF2 #1

0.9 ±0.1 *

60

USF2 #2

0.9 ±0.1 *

60

USF2 #3

1.0 ±0.2 *

67

  1. 1Relative to GAPDH. assayed in triplicate, ±SD, * indicates p < 0.01 compared to negative control.
  2. 2Compared to negative control,
  3. 3RNAi medium GC control from Invitrogen.
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BMC Molecular Biology

ISSN: 1471-2199

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