Figure 6

Effect of RNAi silencing of IRF1, IRF2, USF1 and USF2 on CEACAM1 expression in MDA-MB-468 cells. A. MDA-MB-468 cells were treated with the following controls: U (untreated), lipofectamine (no RNAi), or non-specific RNAi (-) and IRF1, IRF2, USF1, and USF2 levels measured by western blot analysis after 72 hrs. Cells treated with RNAi for IRF1 (3 oligos), IRF2 (3 oligos), USF1 (3 oligos), or USF2 (2 oligos) were analyzed for IRF1, IRF2, USF1, and USF2 expression by western blot analysis after 72 hrs. Lysates were probed with antibody to β-actin to ensure equal protein loading in each lane. B. MDA-MB-468 cells were treated with the following controls: U (untreated), lipofectamine (no RNAi), or non-specific RNAi (-) and CEACAM-1 levels measured by western blot analysis after 72 hrs. Cells treated with RNAi for IRF1 (3 oligos), IRF2 (3 oligos), USF1 (3 oligos), or USF2 (2 oligos) were analyzed for CEACAM1 expression by western blot analysis after 72 hrs. Lysates were probed with antibody to β-actin to ensure equal protein loading in each lane.