Figure 3

Direct binding of transcription factors to the CEACAM1 promoter region. Chromatin immunoprecipitations were performed with antibodies to SP1 and SP2 (K-20) (A), USF1 and USF2 (B), and IRF1 and IRF2 (C) in MDA-MB-468, MCF10A and MCF7 cells. Primers amplify the CEACAM1 promoter region. In: input DNA from the ChIPs; IgG, rabbit IgG used as negative control, numbers refer to percent of input. D. Total cellular protein lysates from MDA-MB-468, MCF10A and MCF7 cells were subjected to Western blot and probed with antibodies to SP1, SP2 (K-20), USF1 and USF2; β-actin was used as a loading control. Closed circle, acetylated form of USF1, open circle, phosphorylated form of USF1, asterisk, USF1 (see Corre et al., [30])