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Figure 5 | BMC Molecular Biology

Figure 5

From: A direct comparison of strategies for combinatorial RNA interference

Figure 5

Inhibition of Luciferase reporters by miRNA-embedded shRNAs. (A) Schematic representation the single-gene targeting miRNA-embedded shRNA plasmids, and normalised ratios of the Renilla:Firefly luciferase activity when DF-1 cells were co-transfected with psi-CHK-1 and the indicated RNAi plasmids. (B) Schematic representation the double gene targeting miRNA-embedded shRNA plasmids, and normalised ratios of the Renilla:Firefly luciferase activity when DF-1 cells were co-transfected with either psi-CHK-1 or psi-CHK-2 and the indicated RNAi plasmids. The mock control refers to a reporter plasmid alone transfection. Values are shown as percentages of the non-targeting shRNA as the mean of 4 replicates ± standard error.

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