Figure 4

Inhibition of Luciferase reporters by long hairpin RNAs. (A) Schematic representation the single-gene targeting lhRNA plasmids, and normalised ratios of the Renilla:Firefly luciferase activity when DF-1 cells were co-transfected with psi-CHK-1 and the indicated RNAi plasmids. (B) Normalised ratios of the Renilla:Firefly luciferase activity when DF-1 cells were co-transfected with psi-CHK-1 and an lhRNA featuring a miR-30 loop sequence (U6/lh-miR-1a-1b), or the indicated RNAi plasmids. (C) Schematic representation the double-gene targeting lhRNA plasmids, and normalised ratios of the Renilla:Firefly luciferase activity when DF-1 cells were co-transfected with either psi-CHK-1 or psi-CHK-2 and the indicated RNAi plasmids. The mock control refers to a reporter plasmid alone transfection. Values are shown as percentages of the non-targeting shRNA as the mean of 4 replicates ± standard error.