Figure 3

Inhibition of Luciferase reporters by dual-U6 promoter delivered shRNAs. (A) Schematic representation of the single-gene targeting dual-U6/shRNA plasmids, and normalised ratios of the Renilla:Firefly luciferase activity when DF-1 cells were co-transfected with psi-CHK-1 and the indicated RNAi plasmids (asterisks indicate P < 0.05 compared to equivalent single shRNAs, n = 4, two-tailed unpaired T-tests). (B) Schematic representation of the double-gene targeting dual-U6/shRNA plasmids, and normalised ratios of the Renilla:Firefly luciferase activity when DF-1 cells were co-transfected with either psi-CHK-1 or psi-CHK-2 and the indicated RNAi plasmids. The mock control refers to a reporter plasmid alone transfection. Values are shown as percentages of the non-targeting shRNA as the mean of 4 replicates ± standard error.