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Table 3 Stability of candidate reference genes in sexual difference of the same tissues of Bactrocera dorsalis evaluated by Normfinder.

From: Evaluation of endogenous references for gene expression profiling in different tissues of the oriental fruit fly Bactrocera dorsalis (Diptera: Tephritidae)

Ranking order Gene name Stability value in midgut Gene name Stability value in Malpighian tubules Gene name Stability value in fat body
1 ACT5 0.050 ACT3 0.180 EF1α 0.210
2 α-TUB 0.136 ACT2 0.200 ACT5 0.215
3 ACT2 0.170 α-TUB 0.240 ACT1 0.325
4 ACT3 0.277 EF1α 0.364 α-TUB 0.361
5 EF1α 0.295 β-TUB 0.463 G6PDH 0.398
6 18S 0.385 GAPDH 0.478 ACT2 0.440
7 GAPDH 0.431 ACT5 0.498 18S 0.494
8 β-TUB 0.458 ACT1 0.542 GAPDH 0.512
9 G6PDH 0.601 G6PDH 0.657 ACT3 0.641
10 ACT1 0.657 18S 0.816 β-TUB 0.755