Evaluation of endogenous references for gene expression profiling in different tissues of the oriental fruit fly Bactrocera dorsalis (Diptera: Tephritidae)

Table 3 Stability of candidate reference genes in sexual difference of the same tissues of Bactrocera dorsalis evaluated by Normfinder.

Ranking order	Gene name	Stability value in midgut	Gene name	Stability value in Malpighian tubules	Gene name	Stability value in fat body
1	ACT5	0.050	ACT3	0.180	EF1α	0.210
2	α-TUB	0.136	ACT2	0.200	ACT5	0.215
3	ACT2	0.170	α-TUB	0.240	ACT1	0.325
4	ACT3	0.277	EF1α	0.364	α-TUB	0.361
5	EF1α	0.295	β-TUB	0.463	G6PDH	0.398
6	18S	0.385	GAPDH	0.478	ACT2	0.440
7	GAPDH	0.431	ACT5	0.498	18S	0.494
8	β-TUB	0.458	ACT1	0.542	GAPDH	0.512
9	G6PDH	0.601	G6PDH	0.657	ACT3	0.641
10	ACT1	0.657	18S	0.816	β-TUB	0.755

ISSN: 1471-2199