Evaluation of endogenous references for gene expression profiling in different tissues of the oriental fruit fly Bactrocera dorsalis (Diptera: Tephritidae)

Table 1 Details of the primer pairs used for real-time PCR.

Gene name	GenBank accession number	Primer sequences (forward/reverse)	Amplicon length (bp)	Efficiency (%)	R ²
GAPDH	GU269901	GACGCCTACAAGCCTGACAT GTTGAAGCGGGAATGATGTT	221	103.2	0.996
G6PDH	AB021910	CCTACAAACTTCTGCGGTTATGC AGAGCGAGGCGAGGTGATC	382	87.2	0.998
EF1α	GU269900	CGTTGGTGTCAACAAGATGG TGCCTTCAGCATTACCTTCC	230	107.6	0.957
18S	AF033944	GCGAGAGGTGAAATTCTTGG CGGGTAAGCGACTGAGAGAG	191	100.3	0.999
α-TUB	GU269902	CGCATTCATGGTTGATAACG GGGCACCAAGTTAGTCTGGA	184	104.8	0.994
β-TUB	EU938673	TTACATTTCTTTATGCCTGGTTTC CATTTGTTCGTCCACTTCCTTC	204	104.0	0.985
ACT1	L12253	AGCGTGAAATCGTGAGGGA GACAAGACCGAGTTGGCATA	286	98.2	0.999
ACT2	L12254	GTGTGATGGTTGGTATGGGA GGCTGGGGAGTTGAAGGTTT	269	89.5	0.998
ACT3	L12255	GGTCGGTATGGGACAGAAGG CTCACGATTGGCTTTTGGAT	220	94.5	0.996
ACT5	L12256	CAACTCACCCGCAATGTATG CGCTCAGCAGTGGTTGTAAA	237	100.6	0.996

ISSN: 1471-2199