Table 4 Cloning parameters for candidate reference genes*
Gene | Primers (5 μM) | Accession number | Anneal. temp. (°C) | Frag. size (bp) | Seq. identity (ref. species, accession number) |
|---|---|---|---|---|---|
eef1 ‡ | FW: atgggaaaggaaaagatccacatca RV: tcaagcttcttgccagaacgacggt | HM367094 | 52 | 1163 | 99% (A. anguilla, EU407825.1) |
actb | FW: agagctacgagctgcctgac RV: cgggtggggcaataatct | HM357464 | 60 | 288 | 92% (A. Anguilla, DQ493907.1) |
odc1 | FW: caratgatgacnttygayws RV: ccrtcrttnacrtartacat | HM357466 | 60 | 581 | 84% (Salmo salar, BT044794.1) |
18s | FW: gtacacacggccggtacagt RV: ggtaggcgcagaaagtacca | FM946133 | 60 | 302 | 100% (A. Australis, FM946133) |