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Figure 1 | BMC Molecular Biology

Figure 1

From: The use of reference gene selection programs to study the silvering transformation in a freshwater eel Anguilla australis: a cautionary tale

Figure 1

Overall variability of mRNA levels of candidate reference genes in liver (A) and ovary (B). Data are based on the threshold cycle (Ct) values of qPCR of reference gene candidates on liver and ovary samples taken in summer 2009. Box-and-whisker plots denote median, upper and lower quartiles, and 10th and 90th percentiles of data. Dashed lines within bars denote the means. Values above bars denote M-value as calculated by GeNorm as a measure of overall stability. Lower values denote lower transcript abundance variability, and thus suitability as a reference gene.

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