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Table 1 Checklist for authors' of MIQE précis at time of manuscript submission, detailing information about individual parameters associated with each step of the RT-qPCR workflow.

From: MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

Sample/Template details
Source If cancer, was biopsy screened for adjacent normal tissue?
Method of preservation Liquid N2/RNAlater/formalin
Storage time (if appropriate) If using samples >6 months old
Handling fresh/frozen/formalin
Extraction method TriZol/columns
RNA: DNA-free Intron-spanning primers/no RT control
Concentration Nanodrop/ribogreen/microfluidics
RNA: integrity Microfluidics/3':5' assay
Inhibition-free Method of testing
Assay optimisation/validation  
Accession number RefSeq XX_1234567
Amplicon details exon location, amplicon size
Primer sequence even if previously published
Probe sequence* identify LNA or other substitutions
In silico BLAST/Primer-BLAST/m-fold
empirical primer concentration/annealing temperature
Priming conditions oligo-dT/random/combination/target-specific
PCR efficiency dilution curve
Linear dynamic range spanning unknown targets
Limits of detection LOD detection/accurate quantification
Intra-assay variation copy numbers not Cq
RT/PCR  
Protocols detailed description, concentrations, volumes
Reagents supplier, Lot number
Duplicate RT ΔCq
NTC Cq & melt curves
NAC ΔCq beginning:end of qPCR
Positive control inter-run calibrators
Data analysis  
Specialist software e.g., QBAsePlus
Statistical justification e.g., biological replicates
Transparent, validated normalisation e.g., GeNorm summary
  1. *Disclosure of probe sequences is strongly encouraged.