Gene symbol | Primer sequence (5' → 3') Forward/Reverse | Amplicon length (bp) | Annealing temperatures (°C) | Amplification efficiency |
---|---|---|---|---|
18S | TGACGGAGAATTAGGGTTCG/ CCGTGTCAGGATTGGGTAAT | 100 | 60 | 1.860 |
ACT2 | TCCACCATGTTCCCTGGTAT/ ACCTCCCAATCCAGACACTG | 124 | 60 | 1.935 |
EF2 | GCGTTCCCTCAGTGTGTCTT/ GGTCATCTGCTCCTTCAAGC | 126 | 60 | 1.790 |
EUC12 | GCGTGGTTCTTGGATCACTA/ TGGTGACAAAGTCAGGTGCT | 114 | 59 | 1.807 |
H2B | GAAGAAGCGGGTGAAGAAGA/ GGCGAGTTTCTCGAAGATGT | 145 | 60 | 1.972 |
IDH * | CTGTTGAGTCTGGAAGATGAC/ CATTTAATTCCTCCCCAACAAA | 271 | 60 | 1.892 |
UBI | AGAAGGAATCGACCCTCCAC/ CCTTGACGTTGTCAATGGTG | 126 | 60 | 1.888 |
SAND | CCATTCAACACTCTCCGACA/ TGTGTGACCCAGCAGAGTAAT | 143 | 59 | 1.919 |
TIP41 | GAACAAAAGCTCGGGACATC/ CAACCAGCAAGAGCATCAAA | 122 | 60 | 1.938 |
TUA | ACCGGTTGATCTCTCAGGTG/ TAAGGGACCAGGTTGGTCTG | 103 | 60 | 1.845 |
33380 | TCCAGAGTGCAATGCTGAAC/ CCCCTCGTCTGGCATACTTA | 134 | 60 | 1.935 |
AGO1 | TCTTGGGCTCGTTTCTCAGT GGAATTCGCCTAGACAGTGC | 144 | 60 | 1.887 |