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Table 1 Description of reference genes used for qPCR in Eucalyptus globulus.

From: Reference gene selection for quantitative reverse transcription-polymerase chain reaction normalization during in vitro adventitious rooting in Eucalyptus globulus Labill

Gene symbol

Gene name

Arabidopsis thaliana's best hit locus

Arabidopsis thaliana locus description

Putative E. globulus orthologs GeneBank accession number

Function

E-value (tblastx)

18S

RNA ribosomal 18S

At3g41768

RNA ribosomal 18S

HO048251

Cytosolic small ribosomal subunit, translation

0,0

ACT2

Actin 2/7

At5g09810

Actin 2/7

HO048249

Structural constituent of cytoskeleton

0,0

EF2

Translation elongation factor 2

At1g56070

Translation elongation factor 2, putative

HO048253

Translation factor activity, nucleic acid binding

e-176

EUC12

Putative RNA bindig protein

*

*

HO048248

Unknown

e-78

H2B

Histone H2B

At5g59910

Histone H2B, putative

HO048243

Strutural constituent of the eukaryotic nucleosome core

e-66

IDH

NADP-Isocitrate dehydrogenase

**

**

HO048252

Carbohydrate metabolism

**

UBI

Polyubiquitin

At4g05050

Polyubiquitin gene, belongs to a subtype group with UBQ10 and UBQ14

HO048245

Signalling complexes for protein degradation, translation control, DNA repair, endocytosis regulation, protein traffic

e-144

SAND

SAND protein

At2g28390

SAND family protein

HO048246

Intracellular vesicular transport, biogenesis and vacuole signalling

e-68

TIP41

TIP41-like protein

At4g34270

TIP41-like family protein

HO048247

Unknown

e-78

TUA

Alpha-tubulin

At5g19780

Alpha tubulin-5

HO048250

Structural constituent of cytoskeleton, microtubule-based processes

e-134

33380

Expressed protein

At4g33380

Expressed sequence

HO048244

Unknown

e-74

  1. * Gene assumed as constitutive in microarray studies employing leaf and vascular tissues of fully grown E. globulus and E. grandis trees (unpublished results). ** Gene obtained from Carvalho et al. (2008) [55].