Table 3 Summary of ATM and ATR mutations detected by HRM.
From: High-resolution melting curve analysis for rapid detection of mutations in a Medaka TILLING library
Gene | Exon | Sequence context | Amino acid change |
|---|---|---|---|
ATM | 1 | 5'-TGAAGCAGGA(T > A)GAGTCTGGCT-3' | M1K |
| Â | 1 | 5'-TGCAGAGGGC(T > G)GGAACATGAT-3' | L15R |
| Â | 1 | 5'-GGCTGGAACA(T > A)GATAAAGCTA-3' | H17Q |
| Â | 1 | 5'-AGCTACTGAG(A > G)GAAAGGTAAA-3' | R23G |
| Â | 2 | 5'-TGAAAACTTC(A > G)GACGGCTCCT-3' | R31G |
| Â | 2 | 5'-AACTTCAGAC(G > A)GCTCCTTCGA-3' | R32Q |
| Â | 2 | 5'-ATGGGACAAT(G > A)TCTTCAGGTA-3' | V62I |
| Â | 9 | 5'-CCACTGCTGT(C > A)GGCGTTGCAC-3' | S444X |
| Â | 9 | 5'-CTGTACGTGC(T > A)GCGCTGCCTC-3' | L463Q |
| Â | 9 | 5'-CGTGCTGCGC(T > A)GCCTCAGGGA-3' | C465S |
| Â | 9 | 5'-CCACACAGAG(T > C)CTCTCAGCCT-3' | S509P |
| Â | 57 | 5'-ACCATGCCCA(T > C)CGGGGAGTTT-3' | I2792T |
| Â | 57 | 5'-ACCTCAGGAT(T > A)GGACCAGCCT-3' | W2812R |
| Â | 58 | 5'-TTGCAAAAAC(T > C)TCAGACCGGT-3' | F2843L |
| Â | 58 | 5'-TGCATGGAAC(G > A)ATTCCTAGAC-3' | R2854Q |
ATR | 12 | 5'-CATGTGAACC(T > A)GACCAGGGAA-3' | L799Q |
| Â | 12 | 5'-CAGGCGGTTC(T > C)CCGTTCTCTG-3' | L810P |
| Â | 12 | 5'-GCGGTTCTCC(G > A)TTCTCTGACC-3' | R811H |
| Â | 13 | 5'-CCTGGTCGCG(C > A)GTCTGAAGGA-3' | R855S |
| Â | 20 | 5'-GGCTTTGACG(A > G)GTGTGATGGC-3' | S1156G |
| Â | 20 | 5'-GTGATGGCTC(T > G)GATGCGCCTG-3' | L1160R |
| Â | 20 | 5'-CGGCCTCCGA(T > C)ACAAAGAAGA-3' | Y1186H |
| Â | 20 | 5'-AAAGAAGACT(T > A)CCCTCTGCTG-3' | F1190Y |
| Â | 24 | 5'-AGCCTGGTGT(C > A)GGTGCTGCTG-3' | S1339X |
| Â | 45 | 5'-CGGCTCCCTT(T > A)GAGGAGAAAC-3' | F2405L |
| Â | 45 | 5'-CATCCGCCGG(T > C)CTTCCATGAG-3' | V2423A |