Table 2 p53 mutations detected by sequencing and HRM.
From: High-resolution melting curve analysis for rapid detection of mutations in a Medaka TILLING library
Sequence context | Type of mutation or amino acid change* | |
|---|---|---|
| Â | SEQ-positive | HRM-positive |
5'-TGGCCCAGTA(T > A)TTTGAAGACC-3' | Y186X | Y186X |
5'-CCGCAGGTAA(A > T)GCTGCCCCAG-3' | Â | Intron |
5'-CTACATGTGT(A > G)ACAGCTCGTG-3' | N220D | Â |
5'-TACATGTGTA(A > G)CAGCTCGTGC-3' | N220S | N220S |
5'-GTGTAACAGC(T > C)CGTGCATGGG-3' | S222P | S222P |
5'-GTGTAACAGCT(C > A)GTGCATGGG-3' | Â | S222X |
5'-TCTGGAAACC(G > T)AGTAAGTTTA-3' | E241X | Â |
5'-GGAAACCGAG(T > C)AAGTTTAGTC-3' | Splice | Â |