Figure 9From: High-resolution melting curve analysis for rapid detection of mutations in a Medaka TILLING libraryRT-PCR amplification of the atm cDNA at different exons. The cDNAs were synthesized from wild-type (atm+/+), atm+/S444X(atm+/-), or atmS444X/S444Xcells (atm-/-) and used as substrates for the PCR amplification of each exon. Left two lanes are DNA size markers, M1: Hind III-digested λ DNA; M2: 100-bp ladder.Back to article page