Figure 1
From: High-resolution melting curve analysis for rapid detection of mutations in a Medaka TILLING library
ENU-induced mutations identified by TILLING in the Oryzias latipes p53 gene (OlP53). (A) Genome organization and protein structure of the medaka p53 gene. The region analyzed by PCR and the HRM assay is indicated by the horizontal bar in the top panel; the protein is shown below. Numbers in the top panel refer to exons; numbers in the bottom panel refer to amino acids. The ENU-induced mutations are shown by arrows. TAD: transactivation domain, DBD: DNA-binding domain, TET: tetramerization domain. High-resolution melting curves (B) and subtraction plots (C) of identified ENU-induced mutations in exons 5 and 6 of the p53 gene in the TILLING genomic DNA.