Figure 6
Repression of CLOCK-BMAL1 activity by wild type but not mutant mCRY2. (A) HEK293T cells were transfected with the pSG-mPer1-Luciferase construct and the indicated wildtype (WT) or mutant mCry2 plasmids. Averages of 3 experiments with SEM are plotted. * p < 0.05, as determined by student's t-test compared to the control (pSG-mPer1-Luc alone). Inset shows analysis of expression levels of WT and mutant CRY2 by western blot analysis. (B) HEK293T cells were transfected with pACT-mCry2 and (C) pACT-mCRY2R501Q/K503R and localizations of encoded proteins were analyzed by using immunocytochemistry with HA antisera and FITC-conjugated secondary antibodies. Both wild type and mutant cryptochromes were localized in the nucleus.