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Figure 5 | BMC Molecular Biology

Figure 5

From: Identification of two Amino Acids in the C-terminal Domain of Mouse CRY2 Essential for PER2 Interaction

Figure 5

Analysis of the interaction between full-length wildtype and mutant mCRY2 with mPER2 using a mammalian two-hybrid system. HEK 293T cells were transfected with pBIND-mPER2/pACT-mCry2 and pACT-mCry2-Mut/pBIND-mPer2 and the pGL5luc reporter plasmid. Top Panel: Expression levels of HA-mCRY2 and FLAG-mPER2. Bottom Panel: Reporter activity was examined 24 h after transfection and the relative luciferase activity was calculated and plotted. Error bars indicate SEM from at least 3 experiments. ** p < 0.005 as determined by student's t-test compared to the control.

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BMC Molecular Biology

ISSN: 1471-2199

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