Figure 3

EGF induces survivin through ERK activation. INS-1 (A) and MIN6 (B) cells were serum-deprived overnight then treated with inhibitors to ERK1/2 (U0126), PI3/AKT (LY294002), STAT3 (AG490), and JNK (SP600125) for 30 minutes prior to the addition of EGF. Proteins were harvested at the indicated times for Western blot analysis and immunoblotted with anti-survivin, anti-actin, and anti-phosphorylated-ERK (p-ERK).