Survival after injection of SeHex and SeSP1 dsRNA. Insect survival rates at different times after injection of dsSeHex, dsSeSP1 and dsGFP. The survival rate was assessed at key developmental stages of 24 h (fifth instar, 3-day-old larvae), 36 h, 48 h (pre-pupae), 60 h (pupae) and 204 h (adult) after injection. Results were arcsine square-root transformed before analysis to correct for the non-normal distribution of percentage values. Different letters at the same detection time indicate a significant difference in survival rate (p < 0.05, Duncan's test). No significant difference was found by ANOVA (p > 0.05). All error bars represent standard deviation (n = 3).