Figure 3

Northern blotting analyses of SeHex and SeSP1 transcript expression in fat body. (A) For Northern blotting analysis, total RNA of day-2 fifth instar S. exigua larvae was extracted from various tissues: brain (Br), cuticle (Cu), fat body (Fb), midgut (Mg), Malpighian tubules (Mt), spermary (Sp) and tracheae (Tr). (B) Developmental expression of SeHex and SeSP1 was analyzed by Northern blotting of fat body from fifth instar larvae to pupae of S. exigua. RNA was extracted from fat body of fifth instar larvae (5L), wandering (pre-pupae) larvae (W) and pupae (P). 5L1-5L3 denoted day-1 to day-3 fifth instar larvae and P1-P7 denote day-1 to day-7 pupae. rRNA was used as a control in all Northern blotting analysis. (C) Developmental expression of SeHex and SeSP1 in young larvae was analyzed by RT-PCR in fat body of S. exigua. RNA was extracted from the fat body of first (1L), second (2L), third (3L), fourth (4L) and fifth instar larvae (5L). L1 means the first day of young larvae, and so on. β-Actin was used as a control in all RT-PCTR analyses.