Figure 2

Phylogenetic analysis of insect hemocyanins based on their amino acid sequences. Full-length amino acid sequences were aligned using Mega 3.1 to generate a phylogenetic tree (Hex, hexamerin; Hex2, class 2 hexamerin gene; SP1, storage protein 1; SP2, storage protein 2). A bootstrap analysis was carried out and the robustness of each cluster was verified in 1000 replications. The hemocyanins were from Aedes aegypti (AaHex2, GALLHP82 and AaSP1, XM_001659481 and AaSP2, XM_001659481), Anopheles gambiae (AgSP1, XM_321800), Apis mellifera (AmHex2, NM_001011600), Apriona germari (AgeSP1, AF509880), Bombyx mori (BmHex2, NM_001044125 and BmSP1, NM_001113276), Camponotus festinates (CfeHex2, AJ251271), Chilo suppressalis (CsSP1, AB248057), Choristoneura fumiferana (CfuSP1, AF007768), Culex quinquefasciatus (CqSP1, XM_001843442 and CqSP2, XM_001843444), Galleria mellonella (GmHex, GALLHP82X;), Heliconius erato (HeSP1, EU711403), Helicoverpa armigera (HaHex, AY661710), Hyalophora cecropia (HceHex, AF032397 and HceSP1, AF032399 and HceSP2, AF032398), Hyphantria cunea (HcuSP1, U60988), Manduca sexta (MsSP1, L07609), Nasonia vitripennis (NvHex2, XM_001606979), Omphisa fuscidentalis (OfHex, EF429085), Periplaneta americana (PaSP1, FM242648), Perla marginata (PmSP1, AM690365), Plodia interpunctella (PiSP1, AF356843), Plutella xylostella (PxSP2, AB266596), Reticulitermes flavipes (RfSP1, AY572858), Reticulitermes speratus (RsSP1, AB371986), Samia cynthia (ScySP1, AB288051), Sesamia nonagrioides (SnSP1, DQ147770), Sinella curviseta (ScuSP1, FM242638), Spodoptera exigua (SeHex, EF646282 and SeSP1, EU259816), Spodoptera litura (SlSP1, AJ249470; SlSP2, AJ249468), Tenebrio molitor (TmSP1, AF395329), Thermobia domestica (TdSP1, FM165290), Tribolium castaneum (TcHex2, XM_968706 and TcSP1, XM_967951) and Trichoplusia ni (TnHex, CBLJHSP and TnSP1, L03280).