Correlation of L1CAM and various transcription factors in EC cell lines. Δct values using β-actin as standard were determined by qRT-PCR analysis in each cell line for L1CAM, Slug, β-catenin and Snail. The EC cell lines included were: Ishikawa, AN3CA, ARK 1, ECC-1, Hec-1A, Hec-1B, Hec-155, IK ER-, MFE 319, RL-95 2, SNG II, SNG M, SPAC-1L, SPAC-1 S. (A) L1CAM versus Slug. (B) L1CAM vs. Snail; (C) L1CAM vs. β-catenin. Note that low Δct values mean high expression of the respective mRNA. A representative experiment from n = 3 is shown.