Chromatin-IP analysis of promoter 1 and promoter 2 sites. Hec-1A (A) and ECC-1 (B) cells were transfected with HA-Slug (left panels) or β catenin (right panels) or an empty vector control (pcDNA3). After 48 h cells were fixed, genomic DNA was isolated sheared for Chromatin-IP using antibodies to β catenin or HA. Liberated DNA was subjected to qRT-PCR analysis using primers specific for all E-boxes or Lef-1 binding sites in the L1CAM promoter 1 or promoter 2 regions. Data ± SD of a representative experiment from n = 3 is shown.