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Table 2 Summary of biochemical assays of the mutants

From: Mutational analysis of an archaeal minichromosome maintenance protein exterior hairpin reveals critical residues for helicase activity and DNA binding

   

ATPase

DNA Binding

 

Mutant No.

Mutant

Oligomeric State

ATPase

Stimulation

ssDNA

Y-DNA

Helicase

WT

-

Hexamer

+++

+++

+++

+++

+++

M2

V324A

Hexamer

++

+++

+++

+++

+++

M3

L325A

Hexamer

++++

++

++++

+++

+

M4

VL324AA

Hexamer

+++

+++

++++

+++

++

M5

E326A

Hexamer

+++

+++

++++

+++

+++

M6

D327A

Hexamer

++

++

+++

+

++

M7

ED327AA

Hexamer

++

++

+

+

+++

M1

R323A

Hexamer

++

+++

++

+

+

M8

R329A

Hexamer

++

++

+

+

+

M9

R331A

Hexamer

0

0

+

+

0

M10

K346A

Hexamer

0

0

+++

++

0

  1. Mutations are grouped by residue type (M2-M4: hydrophobic, M5-M7: acidic, M1, M8-M10: basic). +++: wild type activity level. ++++: greater than wild type (more activity or tighter binding). ++: less than wild type. +: substantially less than wild type. 0: no detectible activity. ATPase stimulation refers to the increase of ATPase activity upon addition of Y-DNA.