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Table 2 Summary of biochemical assays of the mutants

From: Mutational analysis of an archaeal minichromosome maintenance protein exterior hairpin reveals critical residues for helicase activity and DNA binding

    ATPase DNA Binding  
Mutant No. Mutant Oligomeric State ATPase Stimulation ssDNA Y-DNA Helicase
WT - Hexamer +++ +++ +++ +++ +++
M2 V324A Hexamer ++ +++ +++ +++ +++
M3 L325A Hexamer ++++ ++ ++++ +++ +
M4 VL324AA Hexamer +++ +++ ++++ +++ ++
M5 E326A Hexamer +++ +++ ++++ +++ +++
M6 D327A Hexamer ++ ++ +++ + ++
M7 ED327AA Hexamer ++ ++ + + +++
M1 R323A Hexamer ++ +++ ++ + +
M8 R329A Hexamer ++ ++ + + +
M9 R331A Hexamer 0 0 + + 0
M10 K346A Hexamer 0 0 +++ ++ 0
  1. Mutations are grouped by residue type (M2-M4: hydrophobic, M5-M7: acidic, M1, M8-M10: basic). +++: wild type activity level. ++++: greater than wild type (more activity or tighter binding). ++: less than wild type. +: substantially less than wild type. 0: no detectible activity. ATPase stimulation refers to the increase of ATPase activity upon addition of Y-DNA.