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Table 1 Kinetic parameters of EXT-hp mutants

From: Mutational analysis of an archaeal minichromosome maintenance protein exterior hairpin reveals critical residues for helicase activity and DNA binding

  ATpase activity ATpase + Y-DNA ssDNA Binding Y-DNA Binding
Mutant kcat, min-1 km, nM kcat, min-1 km, nM kd, nM Hill kd, nM Hill
WT 3.1 ± 0.1 170 ± 20 3.8 ± 0.1 190 ± 20 240 ± 0.5 8.8 ± 0.5 224 ± 7.3 5.8 ± 1.3
M1 1.8 ± 0.1 140 ± 30 3.7 ± 0.5 440 ± 150 317 ± 5.8 4.4 ± 0.3 398 ± 4.0 13.2 ± 1.3
M2 2.4 ± 0.1 120 ± 20 3.6 ± 0.1 160 ± 20 209 ± 5.8 4.7 ± 0.5 230 ± 7.2 10.1 ± 3.3
M3 4.7 ± 0.3 270 ± 40 5.2 ± 0.2 260 ± 30 169 ± 1.3 5.6 ± 0.1 186 ± 6.4 7.9 ± 1.1
M4 3.9 ± 0.1 230 ± 10 5.3 ± 0.2 260 ± 30 165 ± 10.0 13.4 ± 4.3 178 ± 1.5 7.7 ± 0.5
M5 2.9 ± 0.2 60 ± 40 3.7 ± 0.2 180 ± 30 185 ± 0.3 12.1 ± 0.3 224 ± 0.9 11.6 ± 0.4
M6 2.3 ± 0.1 100 ± 20 2.6 ± 0.2 130 ± 30 283 ± 78.7 15.6 ± 20.1 400 ± 6.5 5.6 ± 0.4
M7 2.0 ± 0.1 90 ± 10 2.2 ± 0.1 90 ± 20 444 ± 1.0 10.6 ± 0.5 404 ± 2.6 8.8 ± 0.4
M8 1.8 ± 0.1 170 ± 40 1.7 ± 0.1 170 ± 30 380 ± 1.8 8.0 ± 0.3 483 ± 0.4 9.0 ± 0.03
M9 No Activity No Activity 325 ± 8.3 6.2 ± 0.8 428 ± 2.0 12.9 ± 0.5
M10 No Activity No Activity 267 ± 7.0 4.5 ± 0.5 339 ± 2.6 11.4 ± 2.0
  1. All values are calculated based on monomeric MCM subunit. For each value, standard error is given. Hill: Hill cooperativity coefficient.