| |
ATpase activity
|
ATpase + Y-DNA
|
ssDNA Binding
|
Y-DNA Binding
|
|---|
|
Mutant
|
kcat, min-1
|
km, nM
|
kcat, min-1
|
km, nM
|
kd, nM
|
Hill
|
kd, nM
|
Hill
|
|---|
|
WT
|
3.1 ± 0.1
|
170 ± 20
|
3.8 ± 0.1
|
190 ± 20
|
240 ± 0.5
|
8.8 ± 0.5
|
224 ± 7.3
|
5.8 ± 1.3
|
|
M1
|
1.8 ± 0.1
|
140 ± 30
|
3.7 ± 0.5
|
440 ± 150
|
317 ± 5.8
|
4.4 ± 0.3
|
398 ± 4.0
|
13.2 ± 1.3
|
|
M2
|
2.4 ± 0.1
|
120 ± 20
|
3.6 ± 0.1
|
160 ± 20
|
209 ± 5.8
|
4.7 ± 0.5
|
230 ± 7.2
|
10.1 ± 3.3
|
|
M3
|
4.7 ± 0.3
|
270 ± 40
|
5.2 ± 0.2
|
260 ± 30
|
169 ± 1.3
|
5.6 ± 0.1
|
186 ± 6.4
|
7.9 ± 1.1
|
|
M4
|
3.9 ± 0.1
|
230 ± 10
|
5.3 ± 0.2
|
260 ± 30
|
165 ± 10.0
|
13.4 ± 4.3
|
178 ± 1.5
|
7.7 ± 0.5
|
|
M5
|
2.9 ± 0.2
|
60 ± 40
|
3.7 ± 0.2
|
180 ± 30
|
185 ± 0.3
|
12.1 ± 0.3
|
224 ± 0.9
|
11.6 ± 0.4
|
|
M6
|
2.3 ± 0.1
|
100 ± 20
|
2.6 ± 0.2
|
130 ± 30
|
283 ± 78.7
|
15.6 ± 20.1
|
400 ± 6.5
|
5.6 ± 0.4
|
|
M7
|
2.0 ± 0.1
|
90 ± 10
|
2.2 ± 0.1
|
90 ± 20
|
444 ± 1.0
|
10.6 ± 0.5
|
404 ± 2.6
|
8.8 ± 0.4
|
|
M8
|
1.8 ± 0.1
|
170 ± 40
|
1.7 ± 0.1
|
170 ± 30
|
380 ± 1.8
|
8.0 ± 0.3
|
483 ± 0.4
|
9.0 ± 0.03
|
|
M9
|
No Activity
|
No Activity
|
325 ± 8.3
|
6.2 ± 0.8
|
428 ± 2.0
|
12.9 ± 0.5
|
|
M10
|
No Activity
|
No Activity
|
267 ± 7.0
|
4.5 ± 0.5
|
339 ± 2.6
|
11.4 ± 2.0
|
- All values are calculated based on monomeric MCM subunit. For each value, standard error is given. Hill: Hill cooperativity coefficient.
