Table 2 Genes used for Real Time RT-qPCR analysis

ACTB

Beta-actin

NM_001101

F = 5'-CTGGAACGGTGAAGGTGACA-3'

R = 5'-AAGGGACTTCCTGTAACAATGCA-3'

Cell motility, structure, integrity

EF1α

Eukaryotic translational elongation factor 1 alpha

NM_001402

F = 5'-AGGTGATTATCCTGAACCATCC-3'

R = 5'-AAAGGTGGATAGTCTGAGAAGC-3'

Translation

B2M

Beta-2-microglobulin

NM_004048

F = 5'-TGCTGTCTCCATGTTTGATGTATCT-3'

R = 5'-TCTCTGCTCCCCACCTCTAAGT-3'

Immune Response: Binds MHC class 1

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

NM_002046

F = 5'-TGCACCACCAACTGCTTAGC-3'

R = 5'-GGCATGGACTGTGGTCATGAG-3'

Metabolism: Glycolysis

[8]

HPRT1

Hypoxanthine phosphoribosyltransferase 1

NM_000194

F = 5'-TGACACTGGCAAAACAATGCA-3'

R = 5'-GGTCCTTTTCACCAGCAAGCT-3'

Nucleotide Salvaging: Purine

synthesis

[8]

*RPL13a

Ribosomal protein L13a

NM_01242

F = 5'-CATAGGAAGCTGGGAGCAAG-3'

R = 5'-GCCCTCCAATCAGTCTTCTG-3'

Translation

*YWHAZ

Tyrosine 3-monooxygenase/tryptophan 5- monooxygenase activation

NM_003406

NM_145690

F = 5'- TGCTTGCATCCCACAGACTA-3'

R = 5'- AGGCAGACAATGACAGACCA-3'

Signal Transduction

UBC

Ubiquitin C

NM_021009

F = 5'-ATTTGGGTCGCGGTTCTTG-3'

R = 5'-TGCCTTGACATTCTCGATGGT-3'

Protein Degradation

[8]

Target Genes

CD31

Platelet/endothelial cell adhesion molecule: PECAM1

NM_000442

F = 5'-AACAGTGTTGACATGAAGAGCC-3'

R = 5'-TGTAAAACAGCACGTCATCCTT-3'

*LTBP2

Latent transforming growth factor binding protein 2

NM_000428

F = 5'-GAGCCCAGCTGGAGTAGGA-3'

R = 5'-AGCTTCTCTGAGTCTAGGGGG-3'

*STC1

Stanniocalcin 1

NM_003155

F = 5'-AGGCAAGGCTGACTTCTCTG-3'

R = 5'-AACTACTTGTCGCATTGGGG-3'

*TSG6

Tumor necrosis factor, alpha- induced protein 6 (TNFAIP6)

NM_007115

F = 5'-TCACATTTCAGCCACTGCTC-3'

R = 5'-TGATCATATCGTCAGTTGTAGTGAA-3'

Rat Specific Normalization Genes

rRPL13a

Ribosomal protein L13a

NM_173340

F = 5'-GGCTGAAGCCTACCAGAAAG-3'

R = 5'-CTTTGCCTTTTCCTTCCGTT-3'

Rat Specific Target Genes

rIGF1

Insulin-like growth factor 1(somatomedin C), transcript variant 1-4

NM_178866

NM_001082477-9

F = 5'- GCTGAAGCCGTTCATTTAGC-3'

R = 5'- GAGGAGGCCAAATTCAACAA-3'

rIGFBP3

Insulin-like growth factor binding protein 3

NM_012588

F = 5'- CTCCATGTGCAGAGATGTCG-3'

R = 5'- CTCTTTTGAAAGCTGCTCC-3'

rIGFBP5

Insulin-like growth factor binding protein 5

NM_012817

F = 5'-AAGGAGACACTCCCCATTCC-3'

R = 5'-TTCCCTTCTCTGTCCGTTCA-3'