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Table 2 Genes used for Real Time RT-qPCR analysis

From: EF1α and RPL13a represent normalization genes suitable for RT-qPCR analysis of bone marrow derived mesenchymal stem cells

Gene Full Name Accession Number Sequence Cellular Function Reference
  Normalization Genes     
ACTB Beta-actin NM_001101 F = 5'-CTGGAACGGTGAAGGTGACA-3'
R = 5'-AAGGGACTTCCTGTAACAATGCA-3'
Cell motility, structure, integrity  
EF1α Eukaryotic translational elongation factor 1 alpha NM_001402 F = 5'-AGGTGATTATCCTGAACCATCC-3'
R = 5'-AAAGGTGGATAGTCTGAGAAGC-3'
Translation  
B2M Beta-2-microglobulin NM_004048 F = 5'-TGCTGTCTCCATGTTTGATGTATCT-3'
R = 5'-TCTCTGCTCCCCACCTCTAAGT-3'
Immune Response: Binds MHC class 1  
GAPDH Glyceraldehyde-3-phosphate dehydrogenase NM_002046 F = 5'-TGCACCACCAACTGCTTAGC-3'
R = 5'-GGCATGGACTGTGGTCATGAG-3'
Metabolism: Glycolysis [8]
HPRT1 Hypoxanthine phosphoribosyltransferase 1 NM_000194 F = 5'-TGACACTGGCAAAACAATGCA-3'
R = 5'-GGTCCTTTTCACCAGCAAGCT-3'
Nucleotide Salvaging: Purine
synthesis
[8]
*RPL13a Ribosomal protein L13a NM_01242 F = 5'-CATAGGAAGCTGGGAGCAAG-3'
R = 5'-GCCCTCCAATCAGTCTTCTG-3'
Translation  
*YWHAZ Tyrosine 3-monooxygenase/tryptophan 5- monooxygenase activation NM_003406
NM_145690
F = 5'- TGCTTGCATCCCACAGACTA-3'
R = 5'- AGGCAGACAATGACAGACCA-3'
Signal Transduction  
UBC Ubiquitin C NM_021009 F = 5'-ATTTGGGTCGCGGTTCTTG-3'
R = 5'-TGCCTTGACATTCTCGATGGT-3'
Protein Degradation [8]
  Target Genes     
CD31 Platelet/endothelial cell adhesion molecule: PECAM1 NM_000442 F = 5'-AACAGTGTTGACATGAAGAGCC-3'
R = 5'-TGTAAAACAGCACGTCATCCTT-3'
  
*LTBP2 Latent transforming growth factor binding protein 2 NM_000428 F = 5'-GAGCCCAGCTGGAGTAGGA-3'
R = 5'-AGCTTCTCTGAGTCTAGGGGG-3'
  
*STC1 Stanniocalcin 1 NM_003155 F = 5'-AGGCAAGGCTGACTTCTCTG-3'
R = 5'-AACTACTTGTCGCATTGGGG-3'
  
*TSG6 Tumor necrosis factor, alpha- induced protein 6 (TNFAIP6) NM_007115 F = 5'-TCACATTTCAGCCACTGCTC-3'
R = 5'-TGATCATATCGTCAGTTGTAGTGAA-3'
  
  Rat Specific Normalization Genes     
rRPL13a Ribosomal protein L13a NM_173340 F = 5'-GGCTGAAGCCTACCAGAAAG-3'
R = 5'-CTTTGCCTTTTCCTTCCGTT-3'
  
  Rat Specific Target Genes     
rIGF1 Insulin-like growth factor 1(somatomedin C), transcript variant 1-4 NM_178866
NM_001082477-9
F = 5'- GCTGAAGCCGTTCATTTAGC-3'
R = 5'- GAGGAGGCCAAATTCAACAA-3'
  
rIGFBP3 Insulin-like growth factor binding protein 3 NM_012588 F = 5'- CTCCATGTGCAGAGATGTCG-3'
R = 5'- CTCTTTTGAAAGCTGCTCC-3'
  
rIGFBP5 Insulin-like growth factor binding protein 5 NM_012817 F = 5'-AAGGAGACACTCCCCATTCC-3'
R = 5'-TTCCCTTCTCTGTCCGTTCA-3'
  
  1. Table#2: Primer pairs preceded by (*) are human species specific primer pairs