Figure 7
Comparison of hTeRT mRNA levels in MIAMI cells, RS-1 cells, and MSC using 3 difference housekeeping genes. MIAMI cells, RS-1 cells, and commercially available MSC were expanded at 21% pO2 or 3% pO2 from passages 1-3. Total RNA was isolated at passage 3 (MIAMI and RS-1) and passage 5 (MSC). RT-qPCR analysis was used to compare the CP levels of 3 housekeeping genes (A) as well as their average standard deviation (B). RT-qPCR analysis was used to compare human Telomerase Reverse Transcriptase (hTeRT) mRNA levels normalized against 1 gene (C: EF1α, RPL13a, GAPDH), or the average of 2-3 genes (D: EF1α and RPL13a or EF1α, RPL13a and GAPDH). Values are shown with standard deviation, with significant differences as p ≤ 0.01 (*) and p ≤ 0.001 (**). N = 3 independent experiments