Figure 6

Detection of species-specific mRNA transcripts in co-cultures of human MIAMI cells injected into rat hippocampal organotypic slices. MIAMI cells were injected into the striatum of rat hippocampal organotypic cultures/slices (RHOS) under oxygen-glucose deprivation conditions [19]. Total RNA was isolated from each organotypic culture containing MIAMI cells. RT-qPCR analysis was completed using 5 ul of undiluted cDNA. All RT-qPCR data using species-specific primer pairs for hSTC1, hTSG6, and hLTBP2 were normalized against both hRPL13a and hYWHAZ (one representative experiment is shown). Rat species-specific RT-qPCR data for rIGIF1, rIGFBP3, rIGFBP5 were normalized against rRPL13a. N = 3 independent experiments.