Comparison of EF1α and RPL13a as a function of oxygen tension. Gene stability of EF1α and RPL13a during expansion of MIAMI cells in different oxygen tensions. Gene stability was determined by comparing the average CP standard deviations for each gene between MIAMI cells expanded at 1, 3, or 21% oxygen for at least 2 passages. The average CP standard deviation was calculated by taking the summation of the CP standard deviations of 4 independent experiments (2-3 data points per experiment) divided by N-1. N = 4 independent experiments.