EF1α and RPL13a have the lowest average CP standard deviation. Determination of the gene stability of 6 potential reference " housekeeping" genes during the expansion of MIAMI cells at 3% pO2. The gene stability was determined by comparing the average CP standard deviations for each gene between experiments. The average CP standard deviation was calculated by taking the summation of the CP standard deviations of 8 independent experiments (2-3 data points per experiment) divided by N-1. N = 8 independent experiments.