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Table 4 Stability value and ranking of reference genes based on NormFinder

From: Determination of reference genes for circadian studies in different tissues and mouse strains

 

Circadian

 

C57BL/6JOlaHsd

129Pas plus C57Bl/6J-KO

129Pas plus C57Bl/6J-WT

Mouse strain

Gene

liver

adrenal gland

liver

adrenal gland

liver

adrenal gland

liver

adrenal gland

Dataset

A

B

C

D

E

F

G

H

Rn18s

0.118

0.070

0.078

0.068

0.063

0.062

0.020

0.054

Actb

0.196

0.090

0.093

0.104

0.087

0.060

0.555

0.506

Gapdh

0.134

0.088

0.123

0.113

0.106

0.058

0.112

0.080

Hmbs

0.096*

0.058

0.114

0.080

0.095

0.063

0.309

0.088

Hprt1

0.150

0.156

0.062

0.166

0.058

0.187

0.024

0.199

Ppib

0.124

0.057*

0.090

0.062*

0.070

0.025*

0.028

0.028*

Rplp0

0.187

0.081

0.056*

0.099

0.077

0.073

0.021

0.064

Eif2a

0.127

 

0.112

 

0.106

 

0.017*

 

Tbcc

0.124

 

0.107

 

0.081

 

0.021

 

Utp6c

0.190

 

0.060

 

0.057*

 

0.027

 

Best two

Ppib

Ppib

Hprt1

Rn18s

Hprt1

Gapdh

Rn18s

Rn18s

 

Hmbs

Hmbs

Ppib

Ppib

Utp6c

Ppib

Eif2a

Ppib

 

0.061

0.046

0.044

0.048

0.037

0.032

0.013

0.031

  1. In groups A to F, data was grouped according to tissue, mouse strain and the time point. In groups G and H, data was grouped only according to tissue and time point. *-best candidate reference gene according to NormFinder. (Best two) - the best combination of two reference genes together with their stability value are calculated only if group identifiers (in our case time of sacrifice) are included in the analysis. "Best two" is not always equal to the first and second ranking genes, but represent the two genes with minimal combined inter-and intragroup expression variation [22].
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BMC Molecular Biology

ISSN: 1471-2199

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