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Table 4 Stability value and ranking of reference genes based on NormFinder

From: Determination of reference genes for circadian studies in different tissues and mouse strains

  Circadian
  C57BL/6JOlaHsd 129Pas plus C57Bl/6J-KO 129Pas plus C57Bl/6J-WT Mouse strain
Gene liver adrenal gland liver adrenal gland liver adrenal gland liver adrenal gland
Dataset A B C D E F G H
Rn18s 0.118 0.070 0.078 0.068 0.063 0.062 0.020 0.054
Actb 0.196 0.090 0.093 0.104 0.087 0.060 0.555 0.506
Gapdh 0.134 0.088 0.123 0.113 0.106 0.058 0.112 0.080
Hmbs 0.096* 0.058 0.114 0.080 0.095 0.063 0.309 0.088
Hprt1 0.150 0.156 0.062 0.166 0.058 0.187 0.024 0.199
Ppib 0.124 0.057* 0.090 0.062* 0.070 0.025* 0.028 0.028*
Rplp0 0.187 0.081 0.056* 0.099 0.077 0.073 0.021 0.064
Eif2a 0.127   0.112   0.106   0.017*  
Tbcc 0.124   0.107   0.081   0.021  
Utp6c 0.190   0.060   0.057*   0.027  
Best two Ppib Ppib Hprt1 Rn18s Hprt1 Gapdh Rn18s Rn18s
  Hmbs Hmbs Ppib Ppib Utp6c Ppib Eif2a Ppib
  0.061 0.046 0.044 0.048 0.037 0.032 0.013 0.031
  1. In groups A to F, data was grouped according to tissue, mouse strain and the time point. In groups G and H, data was grouped only according to tissue and time point. *-best candidate reference gene according to NormFinder. (Best two) - the best combination of two reference genes together with their stability value are calculated only if group identifiers (in our case time of sacrifice) are included in the analysis. "Best two" is not always equal to the first and second ranking genes, but represent the two genes with minimal combined inter-and intragroup expression variation [22].