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Table 2 Primer sequences, exon location (where possible), efficiency and amplicon length for candidate reference genes and the test circadian gene.

From: Determination of reference genes for circadian studies in different tissues and mouse strains

Symbol

Primer

Sequence

Amplicon length

Primer location1 [exon]

Primer efficiency

Reference genes

    

Rplp0

fw

CACTGGTCTAGGACCCGAGAAG

73

4

1.98

 

rv

GGTGCCTCTGGAGATTTTCG

 

4

 

Ppib

fw

GGAGATGGCACAGGAGGAAA

73

3

1.93

 

rv

CCGTAGTGCTTCAGTTTGAAGTTCT

 

4

 

Gapdh

fw

CCAATGTGTCCGTCGTGGATCT

239

5

1.94

 

rv

GTTGAAGTCGCAGGAGACAACC

 

6

 

Actb

fw

CTTCCTCCCTGGAGAAGAGC

124

4

1.98

 

rv

ATGCCACAGGATTCCATACC

 

5

 

Hmbs

fw

TCCCTGAAGGATGTGCCTA

73

7

1.64

 

rv

AAGGGTTTTCCCGTTTGC

 

8

 

Hprt1

fw

TCCTCCTCAGACCGCTTTT

90

1

1.89

 

rv

CCTGGTTCATCATCGCTAATC

 

2

 

Rn18s

fw

CGCCGCTAGAGGTGAAATTC

62

n.a.

1.79

 

rv

TTGGCAAATGCTTTCGCTC

 

n.a.

 

Eif2a

fw

CAACGTGGCAGCCTTACA

74

2

1.95

 

rv

TTTCATGTCATAAAGTTGTAGGTTAGG

 

3

 

Utp6c

fw

TTTCGGTTGAGTTTTTCAGGA

75

17

1.82

 

rv

CCCTCAGGTTTACCATCTTGC

 

18

 

Tbcc

fw

GACTCCTTCCTGAACCTCTGG

62

n.a.

1.90

 

rv

GGAGGCCATTCAAAACTTCA

 

n.a.

 

Circadian gene

    

Dbp

fw

AATGACCTTTGAACCTGATCCCGCT

175

7

1.93

 

rv

GCTCCAGTACTTCTCATCCTTCTGT

 

7

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