Cross-species RNAi using A. avenae EST sequences to induce gene silencing in P. superbus. P. superbus larvae were subjected to RNAi for 11-13 days with selected A. avenae genes (GR463899, ggr-3; GR463913, col-14; GR463914, glutathione peroxidase; and GR463921, novel) identified in a preliminary screen. GFP was taken as a negative control for RNAi. The resulting mixed stage worms were subjected to desiccation at 10% RH for 24 h (negative control - black bar; other samples - grey bars). All samples were also subjected to mock desiccation at 100% RH (white bars). Mean values of triplicates, together with standard deviations, are shown; a one-way ANOVA with Tukey post-hoc test was performed on GR463914 and GR463921: *, significance at P < 0.05; **, significance at P < 0.01; ***, significance at P < 0.001; ns, not significant.