Table 1 Cycle Conditions used for TAIL-PCR
| Reaction |
Program no. | Cycle no. | Thermal condition |
|---|---|---|---|
| Primary (tgp1/ad) | 1 | 1 | 95°C, 5 min |
| 2 | 10 | 94°C, 10 sec; 63°C, 30 sec; 72°C, 3 min | |
| 3 | 1 | 94°C, 10 sec; 25°C, 3 min; 72°C, 2.5 min | |
| 4 | 15 | 94°C, 10 sec; 63°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 63°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 44°C, 1 min; 72°C, 2.5 min | |
| Secondary (tgp2/ad) | 5 | 1 | 95°C, 5 min |
| 6 | 15 | 94°C, 10 sec; 65°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 65°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 44°C, 1 min; 72°C, 2.5 min | |
| Tertiary (tgp3/ad) | 5 | 1 | 95°C, 5 min |
| 6 | 15 | 94°C, 10 sec; 65°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 65°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 44°C, 1 min; 72°C, 2.5 min |