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Table 1 Cycle Conditions used for TAIL-PCR

From: Homologous illegitimate random integration of foreign DNA into the X chromosome of a transgenic mouse line

Reaction Program
no.
Cycle no. Thermal condition
Primary (tgp1/ad) 1 1 95°C, 5 min
  2 10 94°C, 10 sec; 63°C, 30 sec; 72°C, 3 min
  3 1 94°C, 10 sec; 25°C, 3 min; 72°C, 2.5 min
  4 15 94°C, 10 sec; 63°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 63°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 44°C, 1 min; 72°C, 2.5 min
Secondary (tgp2/ad) 5 1 95°C, 5 min
  6 15 94°C, 10 sec; 65°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 65°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 44°C, 1 min; 72°C, 2.5 min
Tertiary (tgp3/ad) 5 1 95°C, 5 min
  6 15 94°C, 10 sec; 65°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 65°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 44°C, 1 min; 72°C, 2.5 min