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Table 1 Cycle Conditions used for TAIL-PCR

From: Homologous illegitimate random integration of foreign DNA into the X chromosome of a transgenic mouse line

Reaction

Program

no.

Cycle no.

Thermal condition

Primary (tgp1/ad)

1

1

95°C, 5 min

 

2

10

94°C, 10 sec; 63°C, 30 sec; 72°C, 3 min

 

3

1

94°C, 10 sec; 25°C, 3 min; 72°C, 2.5 min

 

4

15

94°C, 10 sec; 63°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 63°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 44°C, 1 min; 72°C, 2.5 min

Secondary (tgp2/ad)

5

1

95°C, 5 min

 

6

15

94°C, 10 sec; 65°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 65°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 44°C, 1 min; 72°C, 2.5 min

Tertiary (tgp3/ad)

5

1

95°C, 5 min

 

6

15

94°C, 10 sec; 65°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 65°C, 3 min; 72°C, 2.5 min; 94°C, 10 sec; 44°C, 1 min; 72°C, 2.5 min