Transgene analysis. (A) PCR and (B) Southern blot analysis of total DNA from wild-type C57 (wt), mouse-1 (lane 1) and mouse-2 (lane 2) shows that mouse-1 was fad2 transgenic, whereas mouse-2 was nontransgenic. P, the mixture of fad2 plasmid and wt genomic DNA (A) or the 1036-bp fragments of fad2 transgene digested by EcoR I and Sca I enzymes together (B). (C) PCR analysis of total DNA from 50 heterogeneous F1 mice produced by natural mating of the C57 females and mouse-1 (male) show that all 27 F1 females (mice nos. 1-5, 15-24, 32-36, 39-41, and 44-47) were transgenic, whereas all 23 F1 males (mice nos. 6-14, 25-31, 37-38, 42-43, and 48-50) were nontransgenic. M, DNA marker. P, the mixture of fad2 plasmid and wt genomic DNA.