Quantification of a cDNA dilution series. Template amounts were calculated by applying the different quantification approaches and expressed relative to the undiluted sample. Average values ± standard errors for all tested amplicons are given. ddCq, comparative Cq method; L&S aEff, Liu & Saint (2002) method using average amplification efficiencies for each gene; L&S iEff, Liu & Saint (2002) method using individual amplification efficiencies for each reaction. n = 3.