Figure 3From: Experimental comparison of relative RT-qPCR quantification approaches for gene expression studies in poplarValidation of the comparative Cq method. Different amounts of cDNA were amplified using primer pairs for ACT, EF1and UBQ (reference genes), and APX and SOD (target genes). The ΔCq (Cq target - Cq reference) was plotted against the cDNA input, (A) APX, (B) SOD. n = 3.Back to article page