TY - JOUR AU - Regier, Nicole AU - Frey, Beat PY - 2010 DA - 2010/08/11 TI - Experimental comparison of relative RT-qPCR quantification approaches for gene expression studies in poplar JO - BMC Molecular Biology SP - 57 VL - 11 IS - 1 AB - RT-qPCR is a powerful tool for analysing gene expression. It depends on measuring the increase in fluorescence emitted by a DNA-specific dye during the PCR reaction. For relative quantification, where the expression of a target gene is measured in relation to one or multiple reference genes, various mathematical approaches are published. The results of relative quantification can be considerably influenced by the chosen method. SN - 1471-2199 UR - https://doi.org/10.1186/1471-2199-11-57 DO - 10.1186/1471-2199-11-57 ID - Regier2010 ER -