Figure 4

Cross-responses of the sequences to different compounds as measured using the GFP reporter system. The sequences originally selected on erythromycin (A), chloramphenicol (B), troleandomycin (C), meta-toluate (D) and homoserine lactone (E) were tested. The expression of GFP marker protein was measured 3 h after induction with IPTG in the absence and presence of the compound (E-erythromycin, C-chloramphenicol, T-troleandomycin, M-meta-toluate, H-homoserine lactone). The activity of each construct is shown as a ratio of GFP fluorescence measured in the absence and presence of the compound. Gray bars indicate the activity in the presence of the compound which was used for selecting the sequence.